Objectives : This study was performed to examine the anti-allergic and anti-inflammatory effects of Jacho (Lithospermum erythrorhizon).
Methods : Macrophage 264.7 cells were pretreatment, macrophage were incubated with lipopolysaccharide(LPS) 100ng/ml for 12h (TNF-{alpha}, IL-6) or 24h (IL-1beta, IL-10) and media collectred and TNF-{alpha}, IL-6, IL-1{beta}, and IL-10 concentrations in supernatants were each measured by enzyme-linked immunosorbent assay. Concentrations of Jacho used were 50, 100, 250, 500, and 1000{mu}g/ml, and hydrocortisones used were 10-8, 10-7, 10-6, 10-5, and 10-4M.
Results : Jacho showed inhibitory effect on TNF-{alpha} LPS-stimulated macrophage 264.7. The inhibitory effect was most significant in 250{mu}g/ml, and was not in a dose-dependent manner as in the hydrocortisone group Jacho also showed inhibitory effect on IL-6 by LPS-stimulated macrophage 264.7. The inhibitory effect was most significant in 1000{mu}g/mL, and increased in a roughly dose-dependent manner. Jacho and hydrocortisone showed contrary effect on IL-1beta. Jacho obviously increased the expression of IL-1beta, in alt five concentrations, End at the fewest concentration (50{mu}g/ml) the level of IL-1beta, was highest. On the other hand, hydrocortisone was observed to have inhibitory effect on IL-1beta, in all five concentrations. IL-10 was obviously inhibited by Jacho and hydrocortisone respectively in a roughly dose-dependent manner.
Conclusions : By the findings of this experiment. Jacho was observed to have anti-allergic and anti-inflammatory effects through inhibiting pro-inflammatory cytokine TNF-{alpha} and IL-6, and might be one of the effective therapeutic regimens for allergic diseases.
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